Application Notes

Growing cells in µ-channels (AN 03)
PDF (969 KB)
Here we show how to grow cells inside a µ-Slide I. Additionally, cell culture channels and open wells are compared.

Cell culture coating (AN 08)
PDF (82 KB)
Here you will find exact information on how to make a coating on µ-slides yourself

Chemotaxis (AN 14)
PDF (2.4 MB)
Application Note for chemotactical assays using µ-Slide Chemotaxis

Tube Formation (AN 19)
PDF (1.2 MB)
How to setup a tube formation assay with µ-Slide Angiogenesis

Shear stress and shear rates (AN 11)
PDF (699 KB)
Detailed information on shear stress/shear rates and flow rates in our channel slides.

Shear stress and shear rates in µ-Slide y-shaped (AN 18)
PDF (728 KB)
Detailed information on shear stress/shear rates and flow rates in our µ-Slide y-shaped.

HUVECS under perfusion (AN 13)
PDF (2 MB)
How to set up a flow experiment using μ-Slide I and HUVECs

Serial Connection of flow chambers (AN 25)
PDF (189 KB)
Setup protocol for connecting several Luer-Slides to one Fluidic Unit

Chemotaxis 3D (AN 17)
PDF (1.2 MB)
A general protocol for 3D gel assays with µ-Slide Chemotaxis 3D.

3D Chemotaxis Protocol for non-adherent cells in a gel matrix (AN 23)
PDF (435 KB)
This AN provides a specific example protocol for chemotaxis of Dendritic cells in a collagen gel.

Collagen I Gel for 3D Cell Culture (AN 26)
PDF (57 KB)
Fabrication protocol for collagen I gel (bovine) with different cell media

Adenoviral transduction of human cells (AN 28)
PDF (173 KB)
Detailed protocol how to handle recombinant adenoviruses and design an approach to transduce human cells.

Gene transfection (AN 07)
PDF (295 KB)
This example shows how protocols for gene transfection can be easily adapted to the work in cell culture channels

Fluorescence staining (AN 02/09/15)
In these examples we describe how to do immunofluorescence stainings using µ-Slides.
µ-Slide I / PDF (93 KB)
µ-Slide VI / PDF (121 KB)
µ-Slide y-shaped / PDF (164 KB)

Pixel Size (AN 22)
PDF (272 KB)
How to measure and calculate the pixel size of microscopic images.

Avoiding Evaporation (AN 12)
PDF (126 KB)
How to decrease evaporation during cell cultivation using our humidifying chamber Olaf and µ-Slides

Cultivation of Macrophages (AN 20)
PDF (2.6 MB)
A cultivation protocol for a murine macrophage cell line in µ-Slide VI^0.4 and µ-Slide 8 well.

Gradients inside µ-Slide I (AN 01)
PDF (775 KB)
How to establish a concentration profile

Trypsinization in µ-channels (AN 06)
PDF (153 KB)
How to remove adherent grown cells from a µ-channel after cultivation

Co-Cultivation (AN 10)
PDF (94 KB)
Application note for co-cultivation of two different cell types in µ-Slide 2x9 well

Relocating cells (AN 16)
PDF (604 KB)
Description of µ-Dish^35mmgrid-500 with a gridded bottom for relocation of cells

Lipid monolayer (AN 04)
PDF (150 KB)
Description of an uncomplicated and fast preparation of lipid monolayer on uncoated slides