ibidi Standard Surfaces

The growth, development, and signaling of cultured cells depends strongly on the surface onto which the cells have been seeded. One of the advantages of the ibidi µ-Slides is that they can be treated similarly to standard plastic labware.

RAT1 on Collagen IV



NRK on ibiTreat

Tissue Culture Treated Surface – ibiTreat

ibiTreat is a physical surface modification for improved cell adhesion on µ-Slides. The surface is comparable to standard cell culture flasks and Petri dishes. The adhesion of cells to ibiTreat µ-Slides is strong enough to perform flow experiments simulating the physiological shear stress of the blood flow. The surface was tested with a huge range of different cell lines and primary cells.

Collagen IV

Collagen IV is the primary type of collagen found in large, extracellular membrane structures and complex organs. Collagen IV substrates have been tested for a variety of standard cell lines, epithelial, endothelial, nerve, and muscle cells. The µSlides are coated with a mouse Collagen IV.

Poly-L-Lysine (PLL) / Poly-D-Lysine (PDL)

PLL/PDL is a polymer of the amino acid L/D-Lysine. It is one of the most commonly used adhesion substrates suitable for a large variety of cell types. It has been usedespecially for neuronal cultures. Adhesion via this polymer is mediated by an integrin-independent mechanism.

PDL-coated µ-Slides are available on demand only. Delivery time is about three weeks.

Fibronectin

Fibronectin is a glycoprotein widely used in cell culture coatings. It plays an important role in cell surface interactions, which is mediated by the RGD motive. It allows neurite outgrowth and has been used for glial and neural cells.

Due to its short shelf life, Fibronectin coated µ-Slides are available on demand only. Delivery time is about three weeks.

hydrophobic, uncoated

This surface permits no direct cell growth and can be used for your own specific coating procedures or for non adherent cells.

For special elastic surfaces, click here.