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Applications

Cell Based Perfusion Assays Wound Healing & Invasion Chemotaxis Angiogenesis Special Surfaces
Cell Based
Perfusion Assays
Wound Healing
& Invasion
Chemotaxis Angiogenesis Special
Surfaces

General Aspects

Cell Culture & Microscopy Immunofluorescence Live Cell Imaging Screening Homogenous Cell Distribution
Cell Culture
& Microscopy
Immuno-
fluorescence
Live Cell
Imaging
Screening Homogenuos
Cell Distribution
Standard Surfaces Laser Microdissection      
Standard
Surfaces
Laser Microdissection       

 

APPLICATION NOTES

Growing cells in µ-channels
Here we show how to grow cells inside a µ-Slide I. Additionally, cell culture channels and open wells are compared.
PDF (969 KB)

Cell culture coating
Here you will find exact information on how to make a coating on µ-slides yourself
PDF (135 KB)
Chemotaxis
Application Note for chemotactical assays using µ-Slide Chemotaxis
PDF (3 MB)
Gradients inside
µ-Slide I
How to establish a concentration profile
PDF (775 KB)
Relocating cells
Description of µ-Dish35mmgrid-500 with a gridded bottom for relocation of cells.
PDF (604 KB)
Avoiding Evaporation
How to decrease evaporation during cell cultivation using our humidifying chamber Olaf and µ-Slides
PDF (126 KB)
Fluorescence staining
In these examples we describe how to do immunofluorescence stainings using µ-Slides.
µ-Slide I - PDF (96 KB)
µ-Slide VI - PDF (158 KB)
µ-Slide y-shaped - PDF (163 KB)
Lipid monolayer
Description of an uncomplicated and fast preparation of lipid monolayer on uncoated slides
PDF (136 KB)
Gene transfection
This example shows how protocols for gene transfection can be easily adapted to the work in cell culture channels
PDF (74 KB)
Co-Cultivation
Application note for co-cultivation of two different cell types in µ-Slide 2x9 well.
PDF (94 KB)
Trypsinization in
µ-channels
How to remove adherent grown cells from a µ-channel after cultivation.
PDF (153 KB)
Shear stress and shear rates
Here you will find detailed information on shear stress/shear rates and flow rates in our channel slides.
PDF (560 KB)
HUVECS under perfusion
How to set up a flow experiment using μ-Slide I and HUVECs
PDF (261 KB)
MOVIES

Use of µ-Slides

Seeding cells 
mpg, 1.6 MB
Exchange fluids
mpg, 1.9 MB
Creating a concentration gradient
mpg, 1.2 MB
Preparing
µ-Slide Chemotaxis
wmv (16.5 MB)
mov (9.5 MB)
(please use right mouse button to download video to your PC)

Live Cell Imaging

Mitochondria stain
mpg, 5.4 MB
Bleaching
mpg, 1.3 MB
(1.3 MB)
DNA
mpg, 1.4 MB