| Cells: |
HepG2
(hepatocellular carcinoma) |
| Medium: |
DMEM
supplemented with 10% fetal calf serum (FCS) |
| CO2: |
5% |
| Temperature: |
37°C |
| Humidity: |
75% |
| |
|
| Transfection reagent: |
Metafectene µ FluoR |
| Vector: |
pCMV-GFP |
| Protocol: |
following the instructions on the homepage |
| |
|
| Time intervall: |
15 min (5h) |
| Time intervall: |
10 min (25h, after a medium exchange) |
| Totally elapsed time: |
30 hours |
| Magnification: |
20x |
|
Pictures taken by Helga Wagner and Markus Willemeit on the Nikon Eclipse TiE microscope using the ibidi heating stage and µ-Slide VI 0.4.
Download high-resolution flash video:
MV_012_Transfection_FluoR.flv (1.5 MB)
Download high-res. quicktime-movie:
MV_012_Transfection_FluoR.mov (4.2 MB)
|
