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µ-Slide Chemotaxis

µ-Slide Chemotaxis

µ-Slide for stable gradients, 3 chambers on one slide for chemotaxis and migration assays

Instructions (PDF, 962 KB)
Coating Procedure (PDF, 82 KB)
Application Note for chemotactical assay (PDF, 3 MB)
Chemotaxis and Migration Tool based on ImageJ
Video: Preparing µ-Slide Chemotaxis
wmv format (16.5 MB)  mov format (9.5 MB)
(please use right mouse button to download video to your PC)
Product Information (PDF, 1.5 MB)

  • long term chemotaxis experiments with adherent cells
  • ready to use system, no assembling
  • linear gradients, stable for over 48 hours
  • 3 chambers on one slide for parallel working
  • adapters for different pipettes
  • made for high-end video microscopy

basic principle:

two large reservoirs of 40µl are connected by a narrow observation area

the cells in the observation area become super-imposed by a linear and time-stable gradient


Example experiment

 


  technical details:
chemotaxis chambers on slide 3
volume per chamber 80 µl
observation area 2x1 mm2
total height with plugs 12 mm
volume chemoattractant 18 µl

bottom matches coverslip

No. 1.5

catalog numbers:
ibiTreat, tissue culture treated, sterile
80306
coated (Collagen IV), sterile
80302
hydrophobic, uncoated, sterile
80301

µ-Slide Chemotaxis