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µ-Slide III 0.1

µ-Slide III0.1

3 assays on one µ-Slide for perfusion with rare samples
such as cells or blood

Instructions (PDF, 247 KB)
Shear stress and shear rates (PDF, 699 KB)

Product Flyer Perfusion Assays (PDF, 1.2 MB)

Movie: The ibidi Pump System (MV 20)
Movie: Long Term Cell Culture under Perfusion (MV 21)


Applications

  • mouse blood experiments
  • rolling and adhesion assay
  • long term cultures of cells under perfusion
 

Technical features

  • channel cross section of 0.1 mm (height) x 1 mm (width)
  • small sample amounts
  • 1 mm scale for sample location
  • low dead volume
  • 3 parallel assays on one slide
  • also available as flow kit



dyne/cm² µl/min
1 9.37
10 93.69
100 936.94

Low flowrate for
physiological shear stress
Example experiment and reagent consumption

468 µl sample consumption only, in a one-way setup
for following experimental parameters:

  • shear stress 10 dyne / cm²
  • duration 5 min = 300 sec
  • viscosity 0.01 (dyne sec) / cm²

2 ml minimal sample volume for circular flow setup
using the ibidi perfusion system 

 
  technical details:
adapters female Luer
recommended volume per resservoir 60µl
number of channels 3
volume of each channel 4.5µl
height of channels 0.1mm
length of channels 45mm
width of channels 1mm
growth area per channel 0.43cm²
distance of scale bars 1mm
catalog numbers:
coated (Collagen IV) / 15 pcs. 80332
coated (Fibronectin)* / 15 pcs. 80333
hydrophobic, uncoated / 15 pcs. 80331

* available on request only
µ-Slide III 0.1
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