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ibidi Pump System

ibidi Pump System

For long term unidirectional and/or oscillating flow assays

Instruction Manual (PDF, 2.5 MB)
Instruction Manual Software (PDF, 872 KB)
PumpControl software

Product Information (PDF, 1.3 MB)

Product Flyer Perfusion Assays (PDF, 1.2 MB)

Application Note: HUVECS under perfusion (AN13) (PDF, 2 MB)

Movie: The ibidi Pump System (MV 20)
Movie: Long Term Cell Culture under Perfusion (MV 21)

How to find your pumping system


Applications

  • cultivation of cells under flow conditions, inside an incubator in long term assays
  • cell culture under defined shear stress
  • enrichment of metabolic products as soluble factors
  • long term flow experiments with a minimal amount of medium and supplement
  • endothelial cell interaction with suspended cells under perfusion conditions
 

Technical features

  • unidirectional, continuous flow
  • oscillating flow possible
  • pulsatile flow possible
  • small volume / circular system
  • perfectly compatible with microscopy
  • perfectly compatible with incubator conditions
  • minimal mechanical stress on suspended cells
  • up to four parallel Fluidic Units per air pump
  • fully controllable via PC

Cultivation under flow

Cultivation of endothelial cells under perfusion reflects their natural environment far better than doing so under static conditions. ibidi developed a perfusion system to address exactly that question. The system consists of two main components: a control and a Fluidic Unit. The major advantage of that “split” approach is that the closed flow setup can be assembled and after cultivation transferred to the microscope – without compromising the sterility of the system. The open architecture with the Luer connectors allows the use of any kind of flow devices.


          

The ibidi perfusion system

The control unit is a computer controlled air pump. It generates pressure ranging from -100 to 100 mbar with +/- 1 mbar accuracy. Due to the fact that the flow is generated via air pressure, the mechanical stress on suspended cells in the reservoirs is minimized.The software is a convenient and flexible tool to control the pressure and in turn the shear rate. The Fluidic Unit contains two reservoirs for the cell media, a slide holder and all required tubes for connecting reservoirs and µ-Slides. ibidi offers a range of Perfusion Sets with different diameters and lengths. Therefore these Perfusion Sets allow for varying the flow rates as well as placing the Fluidic Unit on the microscope stage. A set of valves generates an unidirectional flow.

The control unit is connected to the Fluidic Unit by a thin, low-voltage electric cable and a tube for transducing the air pressure which drives the flow. The air tube and the electric cable can be mounted into the incubator, either through a hole in the back wall, or alternatively through the front door seal.

 
 
technical details:
flow rate range
0.03-35 ml/min
shear stress
0.3-150 dyn/cm2
working volume
2.5 / 12 ml
suitable for all µ-Slides with Luer adapters


catalog numbers:
complete ibidi Pump System 10902
ibidi air pressure pump 10905
Fluidic Unit 10903
notebook 10908
 
Perfusion Set, 15 cm, ID 0.5 mm, 2 ml (3 units) 10965
Perfusion Set, 15 cm, ID 0.8 mm, 10 ml (3 units) 10961
Perfusion Set, 15 cm, ID 1.6 mm, 10 ml (3 units) 10962
Perfusion Set, 50 cm, ID 0.5 mm, 2 ml (3 units) 10966
Perfusion Set, 50 cm, ID 0.8 mm, 10 ml (3 units) 10963
Perfusion Set, 50 cm, ID 1.6 mm, 10 ml (3 units) 10964
Reservoir set, 10ml, sterile (10 units) 10971
Reservoir set, 2 ml, sterile (10 units) 10972

 

 

Experimental setup
in an incubator

The system is a fully integrated solution. Hosting the Fluidic Unit in the incubator still allows for running perfusion assays directly at the microscope. A defined temperature and CO2 concentration is assured as the fluidic reservoirs are inside the incubator at all times.
 

Principle of flow generation

Flow in the µ-Slide channels is generated by applying pressured air to the reservoirs of the Fluidic Unit. In order to avoid waste of medium the liquid is pumped back and forth from reservoir (A) to reservoir (B) by switching the four-fold valve (V1).

In state 1 the pressured air is guided to reservoir (A) while reservoir (B) is connected to the ambient air pressure. In state 2 it is the other way around. Switching between these states creates a flow of medium between the reservoirs and ensures that they do not run dry. However, to generate a unidirectional flow a fluidic rectifier is placed between the reservoirs and the µ-Slide. The rectifier is a pinch valve (V2) which clamps off two branches of the Perfusion Set while the others are kept open. The synchronous switching of both valves creates a continuous and unidirectional flow without waste of media.
 

Flow is a parameter


Advantages

  • Fluidic Unit can be placed into the incubator while the control unit is outside the incubator
  • Fluidic Unit and control unit can be separated without losing sterility; makes preparation and live observation on the microscope easy
  

 

  • compatible with all incubators
  • minimized mechanical stress on suspended cells such as monocytes
  • unidirectional flow using small amounts of reagents
  • easy handling with disposable flow chambers and Luer connectors

Example of a Perfusion Set-up on a microscope


Perfusion Sets and µ-Slide selection

    Perfusion Set blue (#10961)
Inner diameter: 0.8 mm Tube length: 15 cm
Total working volume: 11.3 ml Dead volume of tubing: 0.5 ml
 
Growth
area [cm²]
Channel
volume [µl]
Flow rate
[ml/min]
Shear stress
[dyn/cm²]
MIN
MAX
MIN
MAX
µ-Slide I 0.2 Luer
2.5
50
0.83
11.53
4.26
59.14
µ-Slide I 0.4 Luer
2.5
100
1.12
15.30
1.47
20.14
µ-Slide I 0.6 Luer
2.5
150
1.13
15.58
0.68
9.36
µ-Slide I 0.8 Luer
2.5
200
1.15
15.91
0.40
5.53
µ-Slide VI 0.4
0.6
30
1.12
15.25
1.97
26.86
µ-Slide y-shaped
2.8
110
1.08
14.50
2.46
32.98
without slide
-
(75)
1.18
16.51
-
-


    Perfusion Set red (#10962)
Inner diameter: 1.6 mm Tube length: 15 cm
Total working volume: 12.3 ml Dead volume of tubing: 1.5 ml
 
Growth
area [cm²]
Channel
volume [µl]
Flow rate
[ml/min]
Shear stress
[dyn/cm²]
MIN
MAX
MIN
MAX
µ-Slide I 0.2 Luer
2.5
50
1.28
19.11
6.57
98.02
µ-Slide I 0.4 Luer
2.5
100
2.65
33.17
3.49
43.66
µ-Slide I 0.6 Luer
2.5
150
2.74
34.98
1.65
21.02
µ-Slide I 0.8 Luer
2.5
200
2.75
35.14
0.96
12.21
µ-Slide VI 0.4
0.6
30
2.76
35.33
4.86
62.24
µ-Slide y-shaped
2.8
110
2.59
32.12
5.89
73.05
without slide
-
(75)
2.79
35.86
-
-


  Perfusion Set white (#10963)
Inner diameter: 0.8 mm Tube length: 50 cm
Total working volume: 11.7 ml Dead volume of tubing: 0.9 ml
  
Growth area [cm²]
Channel volume [µl]
Flow rate
ml/min]
Shear stress
[dyn/cm²]
MIN
MAX
MIN
MAX
µ-Slide I 0.2 Luer
2.5
50
0.42
7.15
2.15
36.67
µ-Slide I 0.4 Luer
2.5
100
0.57
8.75
0.75
11.52
µ-Slide I 0.6 Luer
2.5
150
0.61
9.40
0.37
5.65
µ-Slide I 0.8 Luer
2.5
200
0.64
10.11
0.22
3.51
µ-Slide VI 0.4
0.6
30
0.63
9.77
1.11
17.21
µ-Slide y-shaped
2.8
110
0.58
8.94
1.32
20.33
without slide
-
(75)
0.61
9.43
-
-


     Perfusion Set yellow/green (#10964)
Inner diameter: 1.6 mm Tube length: 50 cm
Total working volume: 13.6 ml Dead volume of tubing: 2.8 ml
  
Growth area [cm²]
Channel volume [µl]
Flow rate
[ml/min]
Shear stress
[dyn/cm²]
MIN
MAX
MIN
MAX
µ-Slide I 0.2 Luer
2.5
50
1.03
17.00
5.28
87.20
µ-Slide I 0.4 Luer
2.5
100
1.91
25.95
2.51
34.16
µ-Slide I 0.6 Luer
2.5
150
1.98
27.44
1.19
16.49
µ-Slide I 0.8 Luer
2.5
200
2.13
30.25
0.47
10.51
µ-Slide VI 0.4
0.6
30
2.06
28.95
3.63
51.00
µ-Slide y-shaped
2.8
110
1.94
26.60
4.41
60.50
without slide
-
(75)
2.44
36.02
-
-


   Perfusion Set yellow (#10965)*
Inner diameter: 0.5 mm Tube length: 15 cm
Total working volume: 2.5 ml Dead volume of tubing: 0.5 ml
 
Growth area [cm²]
Channel volume [µl]
Flow rate
[ml/min]
Shear stress
[dyn/cm²]
MIN
MAX
MIN
MAX
µ-Slide I 0.1 Luer
2.5
25
0.07
1.35
1.42
27.34
µ-Slide III 0.1
0.43
4.5
0.06
1.08
6.40
115.27
µ-Slide VI 0.1
0.17
1.7
0.08
1.44
8.54
153.69

* Perfusion Set only works with PumpControl version 1.5.0 or higher and
   ibidi Pump with hardware version 1.02 or higher and firmware version 1.10 or higher.


   Perfusion Set black (#10966)*
Inner diameter: 0.5 mm Tube length: 50 cm
Total working volume: 2.7 ml Dead volume of tubing: 0.7 ml
 
Growth area [cm²]
Channel volume [µl]
Flow rate
[ml/min]
Shear stress
[dyn/cm²]
MIN
MAX
MIN
MAX
µ-Slide I 0.1 Luer
2.5
25
0.03
0.61
0.61
12.36
µ-Slide III 0.1
0.43
4.5
0.03
0.52
3.20
55.50
µ-Slide VI 0.1
0.17
1.7
0.03
0.62
3.20
66.17

* Perfusion Set only works with PumpControl version 1.5.0 or higher and
   ibidi Pump with hardware version 1.02 or higher and firmware version 1.10 or higher.

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