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LifeAct

LifeAct-Plasmids

pLifeAct-TagGFP2 | pLifeAct-TagRFP

Novel probe for visualization of actin in eukaryotic cells

Instructions pCMVLifeAct-TagGFP2 (PDF, 313 KB)
Instructions pCMVLifeAct-TagRFP (PDF, 271 KB)
Instructions pCAGLifeAct-TagGFP2 (PDF, 323 KB)
Instructions pCAGLifeAct-TagRFP (PDF, 326 KB)

Product Information (PDF, 289 KB)
Product Flyer (PDF, 636 KB)
Information about TagGFP2 and TagRFP at Evrogen
Safety Data Sheet (PDF, 32 KB)

Movie: LifeAct: Actin Marker for Live Cell Imaging (MV19)


LifeAct, a 17-amino acid peptide, is derived from a protein from Saccharomyces cerevisiae. LifeAct stains filamentous actin (F-actin) structures in living or fixed eukaryotic cells and tissues. In contrast to GFP-actin and variants, LifeAct does not interfere with actin dynamics in vitro and in vivo as shown in several cell types and model organisms.*

After transfection of cells with pLifeAct F-actin is visualized via the fluorescence marker TagGFP2 or TagRFP. Stable transfection of cell lines allows long-term actin staining for various applications.
 

Applications

  • Cytoskeleton Organization:
    -  Non-toxic actin visualization
    -  Fluorescence staining
  • Cytoskeletal Dynamics
    -  Actin dynamics in cellular
       processes
    -  Live cell imaging and microscopy
 

Technical features

  • Brilliant visualization of F-actin
  • Long-term staining with stable cell lines
  • No interference with cytoskeletal dynamics
  • Excellent signal-to-noise ratio
  • Superb biocompatibility

Live cell imaging of actin dynamics in primary dendritic mouse cells, transfected with pCMVLifeAct-TagGFP2

 
 

 

catalog numbers:
pCMVLifeAct-TagGFP2, 20 µg Plasmid
60101
pCMVLifeAct-TagRFP, 20 µg Plasmid  
60102
pCAGLifeAct-TagGFP2, 20 µg Plasmid
60106
pCAGLifeAct-TagRFP, 20 µg Plasmid  
60107

License and product use information

pCMV-LifeAct-TagGFP2 Sequence
pCMV-LifeAct-TagRFP Sequence
pCAG-LifeAct-TagGFP2 Sequence
pCAG-LifeAct-TagRFP Sequence

* Original LifeAct publications in Nature Methods:
Riedl J, Crevenna AH, Kessenbrock K, Yu JH, Neukirchen D, Bista M, Bradke F, Jenne DE, Holak TA, Werb Z, Sixt M & Wedlich-Söldner R. Lifeact – a versatile marker for the visualization of F-actin. Nature Methods 5, 605-607 (2008)
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