Products > Kits & Reagents > LifeAct-Adenoviral Vectors

LifeAct-Adenoviral Vectors

rAV-LifeAct-TagGFP2 | rAV-LifeAct-TagRFP

Novel probe for visualization of actin in eukaryotic cells

Instructions rAV^CMV-LifeAct-TagGFP2 (PDF, 474 KB)
Instructions rAV^CMV-LifeAct-TagRFP (PDF, 507 KB)
Instructions rAV^CAG-LifeAct-TagRFP (PDF, 528 KB)

Product Information (PDF, 259 KB)
Product Flyer (PDF, 636 KB)
Information about TagGFP2 and TagRFP at Evrogen
Safety Data Sheet (PDF, 100 KB)

Movie: LifeAct: Actin Marker for Live Cell Imaging (MV19)

Application Note: Adenoviral transduction of human cells (AN 28)

LifeAct, a 17-amino acid peptide, is derived from a protein from Saccharomyces cerevisiae. LifeAct stains filamentous actin (F-actin) structures in living or fixed eukaryotic cells and tissues. In contrast to GFP-actin and variants, LifeAct does not interfere with actin dynamics in vitro and in vivo as shown in several cell types and model organisms.*

After transduction of cells with rAV-LifeAct F-actin is visualized via the fluorescence marker TagGFP2 or TagRFP. With this method transduction efficiencies can be reached up to 100%, even in difficult-to-transfect cell types like primary cells (e.g. neuronal cells).

Applications

Cytoskeleton Organization:
- Non-toxic actin visualization
- Fluorescence staining
Cytoskeletal Dynamics
-  Actin dynamics in cellular
    processes
-  Live cell imaging and microscopy

Technical features

Brilliant visualization of F-actin
Access to difficult-to-transfect cell lines
No interference with cytoskeletal dynamics
Excellent signal-to-noise ratio
Superb biocompatibility

A549 cells, transduced with rAVCMV-LifeAct-TagGFP2 (green) or rAVCMV-LifeAct-RFP (red)

catalog numbers:

rAV^CMV-LifeAct-TagGFP2, 1x10⁹ IU	60121
rAV^CMV-LifeAct-TagRFP, 1x10⁹ IU	60122
rAV^CAG-LifeAct-TagRFP, 1x10⁹ IU	60124

License and product use information

Please note that you will be working with a product containing infectious virus. Follow the recommended NIH guidelines for all materials containing BSL–2 organisms.

* Original LifeAct publications in Nature Methods:
Riedl J, Crevenna AH, Kessenbrock K, Yu JH, Neukirchen D, Bista M, Bradke F, Jenne DE, Holak TA, Werb Z, Sixt M & Wedlich-Söldner R. Lifeact – a versatile marker for the visualization of F-actin. Nature Methods 5, 605-607 (2008)