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µ-Proteofection Kit VI

µ-Proteofection Kit VI

For direct insertion of antibodies/proteins
into the cytosol of living cells
 

Instructions Proteofection Kit VI, English (PDF, 437 KB)
Instructions Proteofection Kit VI AB, English (PDF, 441 KB)
Instructions Proteofection Kit VI, German (PDF, 435 KB)
Instructions Proteofection Kit VI AB, German (PDF, 439 KB)
Safety Data Sheet Proteofection Kit
Safety Data Sheet Proteofection Kit AB

Applications

  • direct transfer of antibodies/proteins through the membrane
  • protein localization through fluorescent antibodies
  • intracellular competitive inhibition
  • replacing proteins and protein studies in knock down organisms
 

Technical features

  • PROTEOfectene® optimized for protein insertion
  • PROTEOfectene® AB optimized for antibody insertion
  • kits contains PROTEOfectene® (AB), appropriate positive control reagents and µ-Slides VI0.4
  • fast and simple protocol
  • 90 experiments per kit

The PROTEOfectene® reagents (Biontex Laboratories GmbH) were developed to enable the direct uptake of proteins and antibodies through the lipid membrane of cells by endocytosis. So that shuttled proteins reach the cytosol and can act directly in signal transduction. If labeled the fate of the AB / protein can be followed directly via microscopy.


 

  technical details/content:
µ-Slide (ibidi) µ-Slide VI 0.4, ibiTreat (15x)
Reagent (Biontex) PROTEOfectene® (250µl)
Positive control (Biontex) R-Phycoerythrin,
100µg/ml in PBS (100 µl)

µ-Slide (ibidi) µ-Slide VI 0.4, ibiTreat (15x)
Reagent (Biontex) PROTEOfectene® AB (250µl)
Positive control (Biontex) FITC-IgG, 100 µg/ml
in PBS (100 µl)
catalog number:
µ-Proteofection Kit VI 60641

µ-Proteofection Kit VI AB 60642

µ-Proteofection Kit VI