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µ-Transfection Kit VI FluoR

µ-Transfection Kit VI FluoR

For transfection of mammalian cells
with Rhodamine labelled METAFECTENE® µ in µ-Slide VI0.4

Instructions, English (PDF, 1.4 MB)
Instructions, German (PDF, 1.4 MB)
Product Information (PDF, 708 KB)

Application Note: Gene transfection (AN 07) (PDF, 295 KB)
Movie: Transfection in µ-Slide VI 0.4 (MV 12)


  • features similar to µ-Transfection Kit VI but with a fluorescent labeled reagent
  • ideal optical properties for fluorescence microscopy
  • compatible with immersion oil and fixatives


METAFECTENE® µ FluoR (Biontex Laboratories GmbH) is a fluorescence-labelled transfection reagent designed specifically for transfection of mammalian cells using ibidi µ-Slides VI0.4. The Rhodamine labeled compound enables the transfection process to be visualized via tracing of the lipoplex.


Transfection of HeLa cells with µ-Transfection Kit VI FluoR


Light microscopy
image


GFP fluorescence


Lipoplex
fluorescence


Superimposition of
all three images

 
  technical details/content:
µ-Slide (ibidi) µ-Slide VI 0.4, ibiTreat (15x)
Reagent (Biontex) METAFECTENE® µ FluoR (100 µl) (Rhodamine labeled, Excmax 557 nm, Emimax 571 nm)
PBS (Biontex) 1x PBS (10 ml)
catalog number:
µ-Transfection Kit VI FluoR 60602

µ-Transfection Kit VI FluoR