Products > Software and Image Analysis > Chemotaxis and Migration Tool

Chemotaxis and Migration Tool

For data analysis from chemotaxis experiments (time stacks) we developed a free software analysis tool based on NIH‘s ImageJ. This ‘Chemotaxis and Migration Tool‘ runs as a plugin to ImageJ which is freely available and platform independent. Therefore, the software can be run on all major operating systems. The tool provides different types of graphs and statistical tests to perform advanced analysis of experimental data.

After cell tracking, the cells‘ paths can be plotted and analyzed for chemotactical effects. An easy microscopic calibration gives access to a variety of parameters. For specific analyses there are sector tools able to count cells in angular or circular areas, so called Rose plots. Statistical tests complete the software.

All calculations can be directly visualized by histograms and diagrams. Optionally, all data can be exported for further analyses. With a complete set of migrational data the user is able to quantify chemotaxis and random migration.

Together with the ImageJ plugin ‘Chemotaxis and Migration Tool‘ the µ-Slide Chemotaxis enables you to analyze the effect of drugs to the chemotactic behavior of cells in the most detailed manner possible.

Example Data

	Gradient	No Gradient
Total number of cells	76	54
Number of cells moving towards / away from the source	64/12	31/23
Mean average speed of cells non directed	21 µm/hr	16.8 µm/hr
Average cell speed directed	5.3µm/hr	0.85 µm/hr
Directionality	0.31	0.26
X-forward migration index	0.03	-0.01
Y-forward migration index	0.22	-0.04
Rayleigh-test for homogenous distribution (p-value)	p=6.5x10^-12	p=0.22

Downloads:

For data analysis we developed a Chemotaxis and Migration Tool based on ImageJ.
The tool can be downloaded here.

Download ImageJ here: http://rsb.info.nih.gov/ij/

For tracking cells from a time stack we recommend to use Manual Tracking: http://rsb.info.nih.gov/ij/plugins/track/track.html