During which timeframe should I observe the cells when performing a tube formation assay?

The duration of tube formation depends on the cell type and on the extracellular matrix being used, and should be determined individually. Typically, HUVECs already form tubes after 2–4 hours. After 24 hours, the cells start to undergo apoptosis, and this leads to detachment from the matrix and the breaking of the tubes.

Please read our Application Note 27: Tube Formation Data Analysis for more details.

Helpful? (0) (0)