Can the Culture-Inserts be used on protein-coated surfaces? Will the coating be disturbed by pulling out the Culture-Insert?
In general, the Culture-Insert can be placed on top of a coated surface, as long as the coating is dry. Please note that the inserts do not stick to wet surfaces.
However, we highly recommend that you test in advance if insert removal will disrupt the coating in the migration gap. You can analyze this by using a fluorophore-tagged antibody against the coating protein. If the fluorescence signal from the coating in the region of the migration gap is as homogeneous and intense as in the open wells, then you can assume that the coating will stay intact after the insert has been removed. After several successful tests, it is generally safe to perform the actual experiment.
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