Does the gel in the µ-Slide Chemotaxis affect the diffusion of chemoattractant molecules and thereby influence the gradient?
Typical aqueous gels, such as collagen gels or Matrigel®, do not interfere with any diffusion of a chemoattractant molecule in the µ-Slide Chemotaxis. The pore sizes of the gels are too large to affect diffusing molecules. Whenever a gel matrix' pore size is smaller than the diffusion molecules, diffusion will be impaired and a gradient will not be established in a regular way.
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