Is the ibiTreat surface of the µ-Slide Chemotaxis suitable for culturing HUVECs without coating?
HUVECs generally adhere well when using ibidi labware with the ibiTreat (tissue culture-treated) surface, especially, when a growth medium containing 10% fetal calf serum is used. To speed up cell adhesion during chemotaxis assays, however, we recommend using µ-Slides Chemotaxis precoated with Collagen IV. Optionally, it is possible to coat the slides yourself by following the protocol in Application Note 08.
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