My cells do not migrate. What can I do?

Cell migration is a very sensitive process, which is influenced by many factors such as the substrate, coating, as well as the medium and its supplements. If the cells migrate very slowly or do not migrate at all, we suggest to try different experimental conditions, changing only one condition at a time.

The migrating ability also depends strongly on the cell type, cell density, and passage, as all this can influence whether the cells are in a migratory or non-migratory state. In addition, some cell types need special stimuli or have to undergo differentiation before they are able to migrate. Before using a new cell type for a chemotaxis assay, a literature research on these factors is recommended.

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