Which concentration of a chemoattractant should be used?

The concentration of a chemoattractant depends greatly on the used cell type and the experimental setting. For initial experiments, we recommend doing a literature research for the chemoattractant of interest (e.g., publications using transwell assays). If no concentrations have been published, we advise screening a range of concentrations in 10-fold steps (e.g., 1 nM, 10 nM, 100 nM, 1 µM) to identify the most effective chemotaxis result.

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