If all the filling ports on the µ-Slide Chemotaxis chamber are plugged, is it still permeable to CO2 and other gases?
Yes, the polymer material of the µ-Slide Chemotaxis is still gas permeable, even after closing all filling ports with the plugs. This gas permeability, however, is low when the chamber is completely closed. This means that it will take quite a long time for the CO2 (and O2) to seep into the liquid, when the slide is inside the incubator. To avoid air bubbles, we recommend degassing all liquids and the slide before using them by placing them inside the incubator for 24 hours before starting the experiment.
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