Frequently Asked Questions

Chemotaxis Assays

Is imaging of chemotaxis assays only possible with an inverted microscope, or can an upright microscope also be used?

Why are video microscopy and cell tracking needed for the chemotaxis assay? Can’t I simply count migrated cells at the end of the experiment?

Can 3D assays be used for non-adherent cells?

My cells do not migrate. What can I do?

How many cells should be tracked in one observation field?

How long do I have to follow the cell migration with time-lapse microscopy?

Does the gradient change during the chemotaxis experiment?

Which data should be shown when presenting chemotaxis results?

How stable is the gradient over time?

Does the gel in the µ-Slide Chemotaxis affect the diffusion of chemoattractant molecules and thereby influence the gradient?

What is a gradient and what is its difference from a concentration profile in chemotaxis assays?

What is the difference between slice series and track series when using the Chemotaxis and Migration Tool?

Which chemoattractant should be used as a positive control?

If all the filling ports on the µ-Slide Chemotaxis chamber are plugged, is it still permeable to CO2 and other gases?

Which concentration of a chemoattractant should be used?

Which image is used by the Chemotaxis and Migration Tool for calculating the p-value of the Rayleigh test?

I often have trouble loading the observation chamber with the gel mix. How can I improve my method?

Is it possible to do an immunofluorescence staining of cells in a gel matrix using the µ-Slide Chemotaxis?

How can I do a fibronectin coating in the µ-Slide Chemotaxis?

What is the recommended seeding density for non-adherent cells for a chemotaxis assay?

What is the recommended seeding density of adherent cells in a µ-Slide Chemotaxis to conduct a chemotaxis assay?

Do you have any recommendations on how to avoid bubbles that appear after loading cells in the µ-Slide Chemotaxis?

Is the ibiTreat surface of the µ-Slide Chemotaxis suitable for culturing HUVECs without coating?

What is the difference between the Rayleigh test and the Rayleigh test for vector data?

What is the purpose of the Rayleigh test when analyzing chemotaxis assays?

Why do all cell tracks start from one single point in the created plots when using the Chemotaxis and Migration Tool?

How can I plot my chemotaxis data table when using the Chemotaxis and Migration Tool?

Is an analysis with the Chemotaxis and Migration Tool possible when data points of the cell tracks are missing?

Which data format is needed for analyzing chemotaxis assays with the Chemotaxis and Migration Tool?

How do I differentiate directed, chemotactic cell migration from undirected, random migration?

Do you have a movie or other material that illustrates how to use the µ-Slide Chemotaxis?

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